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Diarrhoeagenic E coli PCR kit
EHEC EIEC EPEC ETEC EAEC

The Diarrhoeagenic E. coli PCR Kit (EHEC, EIEC, EPEC, ETEC, EAEC) is an extensive multiplex PCR for in vitro diagnostic PCR detection of diarrhoeagenic E. coli (DEC).

Diarrhoeagenic E. coli PCR kit (EHEC, EIEC, EPEC, ETEC, EAEC)

General

The Diarrhoeagenic E. coli PCR Kit (EHEC, EIEC, EPEC, ETEC, EAEC) is for in vitro diagnostic PCR detection of diarrhoeagenic E. coli (DEC)1. The kit detects the 5 most important diarrhoeagenic groups that are pathogenic for humans; the enterohaemorrhagic E. coli (EHEC), enteroinvasive E. coli (EIEC) , enteropathogenic E. coli (EPEC), attaching and effacing E. coli (A/EEC), enterotoxigenic E. coli (ETEC), and enteroaggregative E. coli (EAEC)2.

The detected groups of diarrhoeagenic E. coli and the genes used to determine them are set out below: 

  • Enterohaemorrhagic strains (EHEC) - identification: demonstration of vtx1 and vtx2.  
  • Enteroinvasive E. coli (EIEC) - identification: demonstration of ipaH.
  • Enteropathogenic E. coli (EPEC) including the attaching and effacing
    E. coli (A/EEC) - identification: demonstration of eae.  
  • Enterotoxigenic E. coli (ETEC) - identification: demonstration of eltA, estA-human, or estA-porcine.  
  • Enteroaggregative E. coli (EAEC) - identification: demonstration of aatA, aggR and aaiC.

The Diarrhoeagenic E. coli PCR Kit (EHEC, EIEC, EPEC, ETEC, EAEC) targets all 5 groups in a single reaction.

Product description

The kit contains all necessary reagents to carry out the DEC PCR reactions.

The kit contains: TE buffer (10 mM Tris-HCL, 1 mM EDTA, pH 8) and 10% Chelex-100 in TE buffer (magnetic stir bar added) to prepare the template, primer mix, PCR Ready Mix (including loading buffer) and two PCR-positive DNA controls.

There is enough reagent for 100 PCR reactions in the kit.

Use

The Diarrhoeagenic E. coli PCR Kit (EHEC, EIEC, EPEC, ETEC, EAEC) is used for in vitro diagnostics of diarrhoeagenic E. coli.

The product can be used in all microbiological laboratories that detect E. coli.

Product range

We also offer the products EAEC PCR KitE. coli vtx1 and vtx2 Subtyping PCR Kit and the DEC PCR Kit in the same product range.

Article
number
Product Number
of tests
Packing 
97004 Diarrhoeagenic E. coli PCR Kit (EHEC, EIEC, EPEC, ETEC, EAEC) 100  1 box 

 

References

1 Persson, S., Olsen, K. E. P., Scheutz, F., Krogfelt, K. A. and Gerner-Smidt, P. 2007. A method for fast and simple detection of major diarrhoeagenic Escherichia coli in the routine diagnostic laboratory. Clin Microbiol Infect. 13:516-524.

2 Nataro, J. P. and J. B. Kaper. 1998. Diarrhoeagenic Escherichia coli. Clin. Microbiol Rev. 11:142-201.

 

Contact

SSI Diagnostica A/S
2 Herredsvejen
3400 Hillerød
Denmark

Tel: +45 4829 9100
Fax: +45 4829 9179

Inquiries:
ssidiagnostica@ssidiagnostica.com
Tel.: +45 4829 9178

Ordering:
ivdorders@ssidiagnostica.com
Tel.: +45 4829 9182

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