SSI DiagnosticaHerredsvejen 23400 HillerødDENMARKT: (+45) 48 29 91 00EAN: 5790 002327452VAT: 37294535
High Quality Salmonella H antisera for slide agglutination and phase inversion.
We market Poly H, H phase pool, H phase, H factor and H:R phase antisera for slide agglutination and H phase antisera for phase inversion on swarm agar plate.Phase inversion antisera are high-titer in contrast to antisera for slide agglutination. Phase inversion antisera are therefore unsuitable for slide agglutination, and antisera for slide agglutination are unsuitable for phase inversion.We market R phase antisera to serotype strains that express R phases.All Salmonella H phase pool antisera are marketed both in 1 mL vials (50 tests) and 3 mL vials (150 tests). All other H sera for slide agglutination are marketed in 3 ml vials (150 tests).Phase inversion sera are marketed in 3 mL vials (30 tests).
When Salmonella H antisera for slide agglutination is used, culture is taken from the edge of the swarm and mixed with approx. 20 μl of antisera.When Salmonella H phase antisera is used for phase inversion, approx. 100 μL of antisera is mixed with 10 mL of swarm agar in a Petri dish (6 cm diameter).Please refer to the product packaging inserts for more details.
The comprehensive range of H antisera for slide agglutination and phase inversion are:
L*: All phases having L1*: 1,2 1,5 1,6 1,7
SSI Diagnostica A/SHerredsvejen 23400 Hillerød
Tel.: +45 4829 9100 (switchboard)
Inquiries:firstname.lastname@example.orgTel.: +45 4829 9178
Frequently asked questions about Salmonella H antisera.
Q: Does the Salmonella antiserum H:2 (art. no. 40340) contain antibodies against antigen H:1,2 or only H:2?A: The antiserum H:2 does not contain antibodies against antigen H:1. The H:1 antibody is removed by absorption. If you observe agglutination with antiserum H:2 you know that the bacterium has antigen combination H:1,2 since H:2 always is together with antigen H:1. If you use antiserum H:1,2 you will have cross reactions to other H:1 combinations (H:1,5, H:1,6 and H:1,7).
Q: When we have an antigen combination with a H:m without H:g we observe a weak agglutination with the antiserum H:g,m (art. no. 23851). Do you have an explanation?A: Yes - When we produce antiserum H:g,m we measure the antibody level using H:g,m and H:m,t expressing strains. This means that the antiserum will agglutinate strongly with H:g,m and H:m,t combinations and probably less strong with many of the other H:g and H:m combinations (i.e. combination H:g,p, H:g,t, H:g,q, H:g,m,s, H:g,z51, H:g,z62, H:g,z63, H:g,s,t, H:f,g,t, H:f,g and H:g,p,u).We always recommend the customers to buy the H phase pool antiserum H:G (art. no. 40299) instead of antiserum H:g,m, and then factor antisera as H:m (art. no. 40332), H:p (art. no. 40333), H:q (art. no. 40334) etc. depending on how much you actually need to know. The reason why we recommend H:G is, that for this antisera we guarantee that the antisera reacts with all H:g combinations and H:m,t. If you then after a positive reaction with antiserum H:G do slide agglutination with the specific factor antisera you will know exactly what antigen combination the Salmonella culture express.
Q: Can I freeze the antibodies?A: Antibodies can be frozen, but the process of freezing and thawing is damaging for the antibodies, and the titer will drop (even though not dramatically) due to decomposition. We have not made further studies on this, and cannot guarantee the stability of the sera, but we have experienced that mostly the titer stays at the same level if a serum only is frozen and thawn once.
Q: What is the definition of titer?A: The titer indicates how many times the serum can be diluted and still give a positive reaction.
Q: How much can the high titer products be diluted?A: The high titer products can be diluted according to the instructions listed on the packaging. The dilution rate varies from product to product, depending on how specific serum, the different rabbits have produced. If the high titer products are diluted more than stated on the packaging, SSI Diagnostica cannot guarantee that the antiserum captures all the wild type strains.
Q: Is it possible to reserve sera from a particular lot?A: Yes, it is possible, if you place an order in advance with the expected total volume for the period. In return we guarantee to keep the reserved lot for you.
Q: Can I order products that do not appear in the catalogue?A: You can always send an inquiry. We might have it. Depending on the needed volume, we might be able to develop the requested product. It can be anything from a new pool composition to a new serum.